LKB1 in Intestinal Epithelial Cells Regulates Bile Acid Metabolism by Modulating FGF15/19 Production.

ABSTRACT:

Background & aims

Liver kinase B1 (LKB1) is a master upstream protein kinase involved in nutrient sensing and glucose and lipid metabolism in many tissues; however, its metabolic role in intestinal epithelial cells (IEC) remains unclear. In this study, we investigated the regulatory role of LKB1 on bile acid (BA) homeostasis.

Methods

We generated mice with IEC-specific deletion of LKB1 (LKB1^ΔIEC) and analyzed the characteristics of IEC development and BA level. In vitro assays with small interfering RNA, liquid chromatography/mass spectrometry, metagenomics, and RNA-sequencing were used to elucidate the regulatory mechanisms underlying perturbed BA homeostasis.

Results

LKB1 deletion resulted in abnormal differentiation of secretory cell lineages. Unexpected

SUBMITTER: Kim Y 

PROVIDER: S-EPMC8873961 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature