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DNA aptamer selection for SARS-CoV-2 spike glycoprotein detection.

ABSTRACT: The rapid spread of SARS-CoV-2 infection throughout the world led to a global public health and economic crisis triggering an urgent need for the development of low-cost vaccines, therapies and high-throughput detection assays. In this work, we used a combination of Ideal-Filter Capillary Electrophoresis SELEX (IFCE-SELEX), Next Generation Sequencing (NGS) and binding assays to isolate and validate single-stranded DNA aptamers that can specifically recognize the SARS-CoV-2 Spike glycoprotein. Two selected non-competing DNA aptamers, C7 and C9 were successfully used as sensitive and specific biological recognition elements for the development of electrochemical and fluorescent aptasensors for the SARS-CoV-2 Spike glycoprotein with detection limits of 0.07 fM and 41.87 nM, respectively.

SUBMITTER: Martinez-Roque MA

PROVIDER: S-EPMC8889740 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature

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DNA aptamer selection for SARS-CoV-2 spike glycoprotein detection.

Martínez-Roque Mateo Alejandro MA Franco-Urquijo Pablo Alberto PA García-Velásquez Víctor Miguel VM Choukeife Moujab M Mayer Günther G Molina-Ramírez Sergio Roberto SR Figueroa-Miranda Gabriela G Mayer Dirk D Alvarez-Salas Luis M LM

Analytical biochemistry 20220302

The rapid spread of SARS-CoV-2 infection throughout the world led to a global public health and economic crisis triggering an urgent need for the development of low-cost vaccines, therapies and high-throughput detection assays. In this work, we used a combination of Ideal-Filter Capillary Electrophoresis SELEX (IFCE-SELEX), Next Generation Sequencing (NGS) and binding assays to isolate and validate single-stranded DNA aptamers that can specifically recognize the SARS-CoV-2 Spike glycoprotein. Tw ...[more]

PMID: 35247355

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