Dataset Information

Transcriptome datasets of β-Aminobutyric acid (BABA)-primed mono- and dicotyledonous plants, Hordeum vulgare and Arabidopsis thaliana.

ABSTRACT: The non-protein amino acid β-Aminobutyric acid (BABA) may trigger the immune responses of plants to various biotic and abiotic stresses leading to a long-term resistance (primed state). We present RNA-seq datasets of BABA - primed mono- and dicotyledonous plants, such as Arabidopsis thaliana and Hordeum vulgare. Illumina NextSeq550 sequencing were carried out after 72 h of BABA exposure. 87 bp long sequence reads were preprocessed of treated and control samples and deposited in the NCBI SRA database. Transcriptome datasets were de novo assembled of each species and deposited in the NCBI TSA database. These SRA and TSA depositions are under the Bioproject accession: PRJNA791573. Pairwise differential expression with enrichment analyses were performed and the most specific DEGs were determined and annotated in both plants.

SUBMITTER: Hegedus G

PROVIDER: S-EPMC8891957 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature

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Transcriptome datasets of β-Aminobutyric acid (BABA)-primed mono- and dicotyledonous plants, Hordeum vulgare and Arabidopsis thaliana.

Hegedűs Géza G Nagy Ágnes Á Decsi Kincső K Kutasy Barbara B Virág Eszter E

Data in brief 20220222

The non-protein amino acid β-Aminobutyric acid (BABA) may trigger the immune responses of plants to various biotic and abiotic stresses leading to a long-term resistance (primed state). We present RNA-seq datasets of BABA - primed mono- and dicotyledonous plants, such as Arabidopsis thaliana and Hordeum vulgare. Illumina NextSeq550 sequencing were carried out after 72 h of BABA exposure. 87 bp long sequence reads were preprocessed of treated and control samples and deposited in the ...[more]

PMID: 35252498

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Project description:Necrotrophic fungi affect a wide range of plants and cause significant crop losses. For the activation of multi-layered innate immune defences, plants can be primed or pre-conditioned to rapidly and more efficiently counteract this pathogen. Untargeted and targeted metabolomics analyses were applied to elucidate the biochemical processes involved in the response of 3,5-dichloroanthranilic acid (3,5-DCAA) primed barley plants to Pyrenophora teres f. teres (Ptt). A susceptible barley cultivar ('Hessekwa') at the third leaf growth stage was treated with 3,5-DCAA 24 h prior to infection using a Ptt conidia suspension. The infection was monitored over 2, 4, and 6 days post-inoculation. For untargeted studies, ultra-high performance liquid chromatography coupled with high-resolution mass spectrometry (UHPLC-MS) was used to analyse methanolic plant extracts. Acquired data were processed to generate the data matrices utilised in chemometric modelling and multi-dimensional data mining. For targeted studies, selected metabolites from the amino acids, phenolic acids, and alkaloids classes were quantified using multiple reaction monitoring (MRM) mass spectrometry. 3,5-DCAA was effective as a priming agent in delaying the onset and intensity of symptoms but could not prevent the progression of the disease. Unsupervised learning methods revealed clear differences between the sample extracts from the control plants and the infected plants. Both orthogonal projection to latent structure-discriminant analysis (OPLS-DA) and 'shared and unique structures' (SUS) plots allowed for the extraction of potential markers of the primed and naïve plant responses to Ptt. These include classes of organic acids, fatty acids, amino acids, phenolic acids, and derivatives and flavonoids. Among these, 5-oxo-proline and citric acid were notable as priming response-related metabolites. Metabolites from the tricarboxylic acid pathway were only discriminant in the primed plant infected with Ptt. Furthermore, the quantification of targeted metabolites revealed that hydroxycinnamic acids were significantly more prominent in the primed infected plants, especially at 2 d.p.i. Our research advances efforts to better understand regulated and reprogrammed metabolic responses that constitute defence priming in barley against Ptt.

Dataset Information

Transcriptome datasets of β-Aminobutyric acid (BABA)-primed mono- and dicotyledonous plants, Hordeum vulgare and Arabidopsis thaliana.

Publications

Transcriptome datasets of β-Aminobutyric acid (BABA)-primed mono- and dicotyledonous plants, <i>Hordeum vulgare</i> and <i>Arabidopsis thaliana</i>.

Similar Datasets

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