Bioanalytics for Influenza Virus-Like Particle Characterization and Process Monitoring.

ABSTRACT: Virus-like particles (VLPs) are excellent platforms for the development of influenza vaccine candidates. Nonetheless, their characterization is challenging due to VLPs' unique biophysical and biochemical properties. To cope with such complexity, multiple analytical techniques have been developed to date (e.g., single-particle analysis, thermal stability, or quantification assays), most of which are rarely used or have been successfully demonstrated for being applicable for virus particle characterization. In this study, several biophysical and biochemical methods have been evaluated for thorough characterization of monovalent and pentavalent influenza VLPs from diverse groups (A and B) and subtypes (H1 and H3) produced in insect cells using the baculovirus expression vector system (IC-BEVS

SUBMITTER: Carvalho SB 

PROVIDER: S-EPMC8894879 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature