Low correlation between Ki67 assessed by qRT-PCR in Oncotype Dx score and Ki67 assessed by Immunohistochemistry.

ABSTRACT: Breast cancers expressing high levels of Ki67 are associated with poor outcomes. Oncotype DX test was designed for ER+/HER2- early-stage breast cancers to help adjuvant chemotherapy decision by providing a Recurrent Score (RS). RS measures the expression of 21 specific genes from tumor tissue, including Ki67. The primary aim of this study was to assess the agreement between Ki67_RNA obtained with Oncotype DX RS and Ki67_IHC. Other objectives were to analyze the association between the event free survival (EFS) and the expression level of Ki67_RNA; and association between RS and Ki67_RNA. Herein, we report a low agreement of 0.288 by Pearson correlation coefficient test between Ki67_IHC and Ki67_RNA in a cohort of 98 patients with early ER+/HER2- breast cancers. Moreover, Ki67_RNA^high tumors were significantly associated with the occurrence of events (p = 0.03). On the other hand, we did not find any association between Ki67_IHC and EFS (p = 0.26). We observed a low agreement between expression level of Ki67_RNA and Ki67 protein labelling by IHC. Unlike Ki67_IHC and independently of the RS, Ki67_RNA could have a prognostic value. It would be interesting to better assess the prognosis and predictive value of Ki67_RNA measured by qRT-PCR. The Ki67_RNA in medical routine could be a good support in countries where Oncotype DX is not accessible.

SUBMITTER: Selmani Z 

PROVIDER: S-EPMC8901910 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature