A novel single-cell RNA-sequencing approach and its applicability connecting genotype to phenotype in ageing disease.

ABSTRACT: Single cell multi-omics analysis has the potential to yield a comprehensive understanding of the cellular events that underlie the basis of human diseases. The cardinal feature to access this information is the technology used for single-cell isolation, barcoding, and sequencing. Most currently used single-cell RNA-sequencing platforms have limitations in several areas including cell selection, documentation and library chemistry. In this study, we describe a novel high-throughput, full-length, single-cell RNA-sequencing approach that combines the CellenONE isolation and sorting system with the ICELL8 processing instrument. This method offers substantial improvements in single cell selection, documentation and capturing rate. Moreover, it allows the use of flexible chemistry for library pr

SUBMITTER: Shomroni O 

PROVIDER: S-EPMC8904555 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature