Silencing of Adc and Ebony Causes Abnormal Darkening of Cuticle in Henosepilachna vigintioctopunctata.

ABSTRACT: N-β-alanyldopamine (NBAD) is a precursor of N-acylquinone sclerotin utilized for cross-linking between cuticular proteins for cuticle during insect molting. The importance of NBAD in cuticle tanning has not been well compared among different developing stages of insects. Henosepilachna vigintioctopunctata, a typical polyphagous pest feeding on a large number of Solanaceae and Cucurbitaceae plants in Asian countries, displays diverse cuticle pigmentation patterns among developing stages and body regions. Here, we found that the expression of three genes (Hvadc, Hvebony, and Hvtan) involved in NBAD biosynthesis peaked in the 4-day-old pupae or 0-day-old adults of H. vigintioctopunctata. At the first, second, third, and fourth larval instar and pupal stage,

SUBMITTER: Ze LJ 

PROVIDER: S-EPMC8907826 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature