Transformation and CRISPR-Cas9-mediated homologous recombination in the fungus Rhizopus microsporus.
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ABSTRACT:
SUBMITTER: Lax C
PROVIDER: S-EPMC8927829 | biostudies-literature | 2022 Mar
REPOSITORIES: biostudies-literature
Publications
Transformation and CRISPR-Cas9-mediated homologous recombination in the fungus <i>Rhizopus microsporus</i>.
Lax Carlos C Navarro-Mendoza María Isabel MI Pérez-Arques Carlos C Navarro Eusebio E Nicolás Francisco Esteban FE Garre Victoriano V
STAR protocols 20220314 1
Here, we describe a reliable approach for targeted DNA integrations in the genome of <i>R. microsporus</i>, one of the main causal agents of mucormycosis. We provide a strategy for stable, targeted integration of DNA templates by homologous recombination (HR) based on the CRISPR-Cas9 technology. This strategy opens a wide range of possibilities for the genetic modification of <i>R. microsporus</i> and will be useful for the study of mucormycosis. For complete details on the use and execution of ...[more]
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