Lacrimal Gland Microenvironment Changes After Obstruction of Lacrimal Gland Ducts.

ABSTRACT:

Purpose

To investigate microenvironment changes of the lacrimal gland after obstruction of lacrimal gland ducts.

Methods

The ducts of rat exorbital lacrimal gland were ligated by sutures for different durations. After that, the sutures in some animals were released, and they were observed for 21 days to evaluate the recovery of the lacrimal gland. Slit lamp and tear secretion test was performed to evaluate ocular surface and lacrimal gland function. The lacrimal gland and cornea were harvested and processed for hematoxylin and eosin staining, oil red O staining, LipidTOX staining, Masson staining, quantitative real time polymerase chain reaction, and immunofluorescence staining.

Results

After the lacrimal gland ducts were blocked, tear secretion and the weight of the

SUBMITTER: He X 

PROVIDER: S-EPMC8934564 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature