CASCADE: Naked eye-detection of SARS-CoV-2 using Cas13a and gold nanoparticles.

ABSTRACT: The COVID-19 pandemic has brought to light the need for fast and sensitive detection methods to prevent the spread of pathogens. The scientific community is making a great effort to design new molecular detection methods suitable for fast point-of-care applications. In this regard, a variety of approaches have been developed or optimized, including isothermal amplification of viral nucleic acids, CRISPR-mediated target recognition, and read-out systems based on nanomaterials. Herein, we present CASCADE (CRISPR/CAS-based Colorimetric nucleic Acid DEtection), a sensing system for fast and specific naked-eye detection of SARS-CoV-2 RNA. In this approach, viral RNA is recognized by the LwaCas13a CRISPR protein, which activates its collateral RNase activity. Upon target recognition, Cas13a clea

SUBMITTER: Lopez-Valls M 

PROVIDER: S-EPMC8939626 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature