β-cell-specific deletion of PFKFB3 restores cell fitness competition and physiological replication under diabetogenic stress.

ABSTRACT: HIF1α and PFKFB3 play a critical role in the survival of damaged β-cells in type-2 diabetes while rendering β-cells non-responsive to glucose stimulation. To discriminate the role of PFKFB3 from HIF1α in vivo, we generated mice with conditional β-cell specific disruption of the Pfkfb3 gene on a human islet pancreatic polypeptide (hIAPP^+/-) background and a high-fat diet (HFD) [PFKFB3^βKO + diabetogenic stress (DS)]. PFKFB3 disruption in β-cells under DS led to selective purging of hIAPP-damaged β-cells and the disappearance of insulin- and glucagon positive bihormonal cells. PFKFB3 disruption induced a three-fold increase in β-cell replication as evidenced by minichromosome maintenance 2 protein (MCM2) expression. Unlike high-, lower DS or switch to restricted chow die

SUBMITTER: Min J 

PROVIDER: S-EPMC8941137 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature