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Protocol for RNA fluorescence in situ hybridization in mouse meningeal whole mounts.

ABSTRACT: The multilayered meninges surrounding the brain and spinal cord harbor distinct immune cell populations with prominent roles in health and diseases. Here we present an optimized protocol for RNA fluorescence in situ hybridization (RNA FISH) in meningeal whole mounts, allowing the visualization of gene expression. We also describe the combination of this protocol with immunohistochemistry for simultaneous visualization of mRNA and proteins. This protocol can be used for assessing spatial gene expression within the meninges.

SUBMITTER: Nilsson OR

PROVIDER: S-EPMC8956821 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature

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Protocol for RNA fluorescence in situ hybridization in mouse meningeal whole mounts.

Nilsson Olof Rickard OR Kari Laszlo L Rosenke Rebecca R Steele-Mortimer Olivia O

STAR protocols 20220322 2

The multilayered meninges surrounding the brain and spinal cord harbor distinct immune cell populations with prominent roles in health and diseases. Here we present an optimized protocol for RNA fluorescence in situ hybridization (RNA FISH) in meningeal whole mounts, allowing the visualization of gene expression. We also describe the combination of this protocol with immunohistochemistry for simultaneous visualization of mRNA and proteins. This protocol can be used for assessing spatial g ...[more]

PMID: 35345596

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