A fluorescence in situ hybridization (FISH) protocol for stickleback tissue.

ABSTRACT:

Background

Threespine stickleback are an important model for behaviour and evolutionary studies. A growing number of quantitative trait loci (QTL) and gene expression studies are identifying genes related to ecologically important traits in sticklebacks. In order to visualize the expression of candidate genes, we developed a fluorescence in situ hybridization (FISH) protocol.

Methods

We present a protocol for FISH on fresh or flash-frozen dissected tissue, using either cryo- or paraffin embedding. The protocol covers probe design guidelines and synthesis, sample embedding, sectioning, and the hybridization process. The protocol is optimized for brain tissue. Key steps for modifying the protocol for other tissues are noted.

Results

The FISH protocol resulted in

SUBMITTER: James N 

PROVIDER: S-EPMC5642962 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature