Dataset Information

Risk factors for the abundance of antimicrobial resistance genes aph(3')-III, erm(B), sul2 and tet(W) in pig and broiler faeces in nine European countries.

ABSTRACT:

Objectives

The occurrence and zoonotic potential of antimicrobial resistance (AMR) in pigs and broilers has been studied intensively in past decades. Here, we describe AMR levels of European pig and broiler farms and determine the potential risk factors.

Methods

We collected faeces from 181 pig farms and 181 broiler farms in nine European countries. Real-time quantitative PCR (qPCR) was used to quantify the relative abundance of four antimicrobial resistance genes (ARGs) [aph(3')-III, erm(B), sul2 and tet(W)] in these faeces samples. Information on antimicrobial use (AMU) and other farm characteristics was collected through a questionnaire. A mixed model using country and farm as random effects was performed to evaluate the relationship of AMR with AMU and other farm characteristics. The correlation between individual qPCR data and previously published pooled metagenomic data was evaluated. Variance component analysis was conducted to assess the variance contribution of all factors.

Results

The highest abundance of ARG was for tet(W) in pig faeces and erm(B) in broiler faeces. In addition to the significant positive association between corresponding ARG and AMU levels, we also found on-farm biosecurity measures were associated with relative ARG abundance in both pigs and broilers. Between-country and between-farm variation can partially be explained by AMU. Different ARG targets may have different sample size requirements to represent the overall farm level precisely.

Conclusions

qPCR is an efficient tool for targeted assessment of AMR in livestock-related samples. The AMR variation between samples was mainly contributed to by between-country, between-farm and within-farm differences, and then by on-farm AMU.

SUBMITTER: Yang D

PROVIDER: S-EPMC8969523 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature

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Publications

Risk factors for the abundance of antimicrobial resistance genes aph(3')-III, erm(B), sul2 and tet(W) in pig and broiler faeces in nine European countries.

Yang Dongsheng D Heederik Dick J J DJJ Mevius Dik J DJ Scherpenisse Peter P Luiken Roosmarijn E C REC Van Gompel Liese L Skarżyńska Magdalena M Wadepohl Katharina K Chauvin Claire C Van Heijnsbergen Eri E Wouters Inge M IM Greve Gerdit D GD Jongerius-Gortemaker Betty G M BGM Tersteeg-Zijderveld Monique M Zając Magdalena M Wasyl Dariusz D Juraschek Katharina K Fischer Jennie J Wagenaar Jaap A JA Smit Lidwien A M LAM Schmitt Heike H

The Journal of antimicrobial chemotherapy 20220301 4

<h4>Objectives</h4>The occurrence and zoonotic potential of antimicrobial resistance (AMR) in pigs and broilers has been studied intensively in past decades. Here, we describe AMR levels of European pig and broiler farms and determine the potential risk factors.<h4>Methods</h4>We collected faeces from 181 pig farms and 181 broiler farms in nine European countries. Real-time quantitative PCR (qPCR) was used to quantify the relative abundance of four antimicrobial resistance genes (ARGs) [aph(3')- ...[more]

PMID: 35061866

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Dataset Information

Risk factors for the abundance of antimicrobial resistance genes aph(3')-III, erm(B), sul2 and tet(W) in pig and broiler faeces in nine European countries.

Objectives

Methods

Results

Conclusions

Publications

Risk factors for the abundance of antimicrobial resistance genes aph(3')-III, erm(B), sul2 and tet(W) in pig and broiler faeces in nine European countries.

Similar Datasets

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