Clonal Transitions and Phenotypic Evolution in Barrett's Esophagus.

ABSTRACT:

Background & aims

Barrett's esophagus (BE) is a risk factor for esophageal adenocarcinoma but our understanding of how it evolves is poorly understood. We investigated BE gland phenotype distribution, the clonal nature of phenotypic change, and how phenotypic diversity plays a role in progression.

Methods

Using immunohistochemistry and histology, we analyzed the distribution and the diversity of gland phenotype between and within biopsy specimens from patients with nondysplastic BE and those who had progressed to dysplasia or had developed postesophagectomy BE. Clonal relationships were determined by the presence of shared mutations between distinct gland types using laser capture microdissection sequencing of the mitochondrial genome.

Results

We identified 5 differen

SUBMITTER: Evans JA 

PROVIDER: S-EPMC8972067 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature