Homologous PB1 gene promotes the replication efficiency of avian influenza H7N4 candidate vaccine virus.

ABSTRACT:

Background

The first and only case of human infection with the avian influenza A (H7N4) virus in China emerged in 2018. The H7N4 virus was distinct from previous H7N9 viruses and raised public concerns. Therefore, developing a suitable H7N4 candidate vaccine virus (CVV) remains crucial for potential pandemic preparedness.

Methods

We constructed a reassortant virus with a (6 + 2) genome composition, then introduced the polymerase basic protein 1 (PB1) from a wild-type virus to develop a (5 + 3) reassortant virus through reverse genetics. We performed whole-genome sequencing to confirm the genome stability, assessed the growth ability in MDCK cells, and analyzed virus antigenicity using hemagglutination inhibition assays. Subsequently, the effect of homologous PB1 on polymeras

SUBMITTER: Liu L 

PROVIDER: S-EPMC8983892 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature