Alteration of N⁶-Methyladenosine mRNA Methylation in a Human Stem Cell-Derived Cardiomyocyte Model of Tyrosine Kinase Inhibitor-Induced Cardiotoxicity.

ABSTRACT:

Background

N⁶-methyladenosine (m⁶A) plays important roles in various cardiovascular diseases (CVDs), including cardiac hypertrophy and heart failure. Sunitinib (SUN) is a tyrosine kinase inhibitor (TKI) that is widely used in the treatment of different types of solid and blood tumors, but its efficacy is restricted by a concomitant rise in cardiotoxicities. However, the methylation modification of m⁶A messenger RNA (mRNA) in cardiomyocytes treated with TKI has not been investigated.

Methods

The global m⁶A methylation level of SUN-induced cardiotoxicity was detected by m⁶A dot blot and colorimetric methylation assay. MeRIP-Seq (methylated RNA immunoprecipitation sequencing) and RNA-seq (RNA sequencing, input) were employe

SUBMITTER: Ma Y 

PROVIDER: S-EPMC8985653 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature