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Directed differentiation of human pluripotent stem cells to epicardial-derived fibroblasts.

ABSTRACT: Cardiac fibroblasts (CFBs) are a key therapeutic target due to their supportive roles during heart development and response to injury and disease. Here, we describe a robust protocol to differentiate human pluripotent stem cells (hPSCs) into CFBs through an epicardial intermediate. We discuss in detail the characterization of the resulting epicardial-derived fibroblasts (EpiC-FBs) using immunofluorescence microscopy, flow cytometry, and qPCR. We anticipate that these EpiC-FBs can be applied to drug testing, disease modeling, and tissue engineering. For complete details on the use and execution of this protocol, please refer to Bao et al. (2016), Floy et al. (2021), and Lian et al. (2015).

SUBMITTER: Floy ME

PROVIDER: S-EPMC8991283 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature

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Directed differentiation of human pluripotent stem cells to epicardial-derived fibroblasts.

Floy Martha E ME Shabnam Fathima F Palecek Sean P SP

STAR protocols 20220406 2

Cardiac fibroblasts (CFBs) are a key therapeutic target due to their supportive roles during heart development and response to injury and disease. Here, we describe a robust protocol to differentiate human pluripotent stem cells (hPSCs) into CFBs through an epicardial intermediate. We discuss in detail the characterization of the resulting epicardial-derived fibroblasts (EpiC-FBs) using immunofluorescence microscopy, flow cytometry, and qPCR. We anticipate that these EpiC-FBs can be applied to d ...[more]

PMID: 35403005

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