Efficiently generate functional hepatic cells from human pluripotent stem cells by complete small-molecule strategy.

ABSTRACT:

Background

Various methods have been developed to generate hepatic cells from human pluripotent stem cells (hPSCs) that rely on the combined use of multiple expensive growth factors, limiting industrial-scale production and widespread applications. Small molecules offer an attractive alternative to growth factors for producing hepatic cells since they are more economical and relatively stable.

Methods

We dissect small-molecule combinations and identify the ideal cocktails to achieve an optimally efficient and cost-effective strategy for hepatic cells differentiation, expansion, and maturation.

Results

We demonstrated that small-molecule cocktail CIP (including CHIR99021, IDE1, and PD0332991) efficiently induced definitive endoderm (DE) formation via increased endogeno

SUBMITTER: Pan T 

PROVIDER: S-EPMC8996222 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature