ClC-K2 Cl^- channel allows identification of A- and B-type of intercalated cells in split-opened collecting ducts.

ABSTRACT: The collecting duct is a highly adaptive terminal part of the nephron, which is essential for maintaining systemic homeostasis. Principal and intercalated cells perform different physiological tasks and exhibit distinctive morphology. However, acid-secreting A- and base secreting B-type of intercalated cells cannot be easily separated in functional studies. We used BCECF-sensitive intracellular pH (pH_i ) measurements in split-opened collecting ducts followed by immunofluorescent microscopy in WT and intercalated cell-specific ClC-K2^-/- mice to demonstrate that ClC-K2 inhibition enables to distinguish signals from A- and B-intercalated cells. We show that ClC-K2 Cl^- channel is expressed on the basolateral side of intercalated cells, where it governs Cl^-

SUBMITTER: Pyrshev K 

PROVIDER: S-EPMC9014849 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature