Construction of Strand-seq libraries in open nanoliter arrays.

ABSTRACT: Single-cell Strand-seq generates directional genomic information to study DNA repair, assemble genomes, and map structural variation onto chromosome-length haplotypes. We report a nanoliter-volume, one-pot (OP) Strand-seq library preparation protocol in which reagents are added cumulatively, DNA purification steps are avoided, and enzymes are inactivated with a thermolabile protease. OP-Strand-seq libraries capture 10%-25% of the genome from a single-cell with reduced costs and increased throughput.

SUBMITTER: Hanlon VCT 

PROVIDER: S-EPMC9017222 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature