Identification of Sodium Transients Through Na_V1.5 Channels as Regulators of Differentiation in Immortalized Dorsal Root Ganglia Neurons.

ABSTRACT: Neuronal differentiation is a complex process through which newborn neurons acquire the morphology of mature neurons and become excitable. We employed a combination of functional and transcriptomic approaches to deconvolute and identify key regulators of the differentiation process of a DRG neuron-derived cell line, and we focused our study on the Na _V 1.5 ion channel (encoded by Scn5a) as a channel involved in the acquisition of DRG neuronal features. Overexpression of Scn5a enhances the acquisition of neuronal phenotypic features and increases the KCl-elicited hyperexcitability response in a DRG-derived cell line. Moreover, pharmacologic inhibition of the Na _V 1.5 channel during differentiation hinders the acquisition of phenotypic featur

SUBMITTER: Martinez AL 

PROVIDER: S-EPMC9018981 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature