Dataset Information

Tanshinone I attenuates fibrosis in fibrotic kidneys through down-regulation of inhibin beta-A.

ABSTRACT:

Background

Tanshinone I (Tan-I), an ingredient of Salvia miltiorrhiza, displays protective effects in several disease models. We aim to study the effect of Tan-I on renal fibrosis and explore its underlining mechanism.

Methods

Rat renal fibroblasts (NRK-49F) were used as an in vitro model to study the effect of Tan-I. Mouse renal fibrosis model was induced by unilateral ureteral obstruction (UUO) or peritoneally injection of aristolochic acid I (AAI).

Results

We found that Tan-I dose-dependently inhibited the expression of pro-fibrotic markers in rat renal fibroblasts. Masson staining and Western blotting analysis showed that Tan-I treatment attenuated renal fibrosis in UUO or AAI induced fibrotic kidneys. RNA sequencing analysis identified inhibin beta-A (INHBA), a ligand of TGF-β superfamily, as a downstream target of Tan-I in fibrotic kidneys, which were further verified by qPCR. Western blotting analysis showed that INHBA is up-regulated in UUO or AAI induced fibrotic kidneys and Tan-I reduced the expression of INHBA in fibrotic kidneys. Inhibition of INHBA by Tan-I was further confirmed in rat fibroblasts. Moreover, knockdown of INHBA reduced the expression of pro-fibrotic markers and abolished the ani-fibrotic effect of Tan-I in rat renal fibroblasts.

Conclusions

We conclude that Tan-I attenuates fibrosis in fibrotic kidneys through inhibition of INHBA.

SUBMITTER: Wu M

PROVIDER: S-EPMC9020026 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature

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Publications

Tanshinone I attenuates fibrosis in fibrotic kidneys through down-regulation of inhibin beta-A.

Wu Ming M Yang Feng F Huang Di D Ye Chaoyang C

BMC complementary medicine and therapies 20220419 1

<h4>Background</h4>Tanshinone I (Tan-I), an ingredient of Salvia miltiorrhiza, displays protective effects in several disease models. We aim to study the effect of Tan-I on renal fibrosis and explore its underlining mechanism.<h4>Methods</h4>Rat renal fibroblasts (NRK-49F) were used as an in vitro model to study the effect of Tan-I. Mouse renal fibrosis model was induced by unilateral ureteral obstruction (UUO) or peritoneally injection of aristolochic acid I (AAI).<h4>Results</h4>We found that ...[more]

PMID: 35439976

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Dataset Information

Tanshinone I attenuates fibrosis in fibrotic kidneys through down-regulation of inhibin beta-A.

Background

Methods

Results

Conclusions

Publications

Tanshinone I attenuates fibrosis in fibrotic kidneys through down-regulation of inhibin beta-A.

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