Colorectal Cancer Is Associated with the Presence of Cancer Driver Mutations in Normal Colon.

ABSTRACT: Although somatic mutations in colorectal cancer are well characterized, little is known about the accumulation of cancer mutations in the normal colon before cancer. Here, we have developed and applied an ultrasensitive, single-molecule mutational test based on CRISPR-DS technology, which enables mutation detection at extremely low frequency (<0.001) in normal colon from patients with and without colorectal cancer. This testing platform revealed that normal colon from patients with and without colorectal cancer carries mutations in common colorectal cancer genes, but these mutations are more abundant in patients with cancer. Oncogenic KRAS mutations were observed in the normal colon of about one third of patients with colorectal cancer but in none of the patients without colorectal cancer.

SUBMITTER: Matas J 

PROVIDER: S-EPMC9022358 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature