Improved liquid-liquid extraction by modified magnetic nanoparticles for the detection of eight drugs in human blood by HPLC-MS.

ABSTRACT: Magnetic nanoparticles modified with porous titanium dioxide were used as clean-up nanospheres for the detection of eight drug poisons in human blood by high-performance liquid chromatography-mass spectrometry. The magnetic clean-up nanospheres (Fe₃O₄@mTiO₂) with a mesoporous structure were successfully synthesized and characterized by scanning electron microscopy/energy dispersive spectroscopy, transmission electron microscopy, X-ray diffractometry, vibrating sample magnetometry, infrared spectroscopy, and Brunauer-Emmett-Teller techniques. Lipid co-extractives, such as phosphatidic acid and fatty acids, which are major interferences in HPLC-MS analysis causing ion suppression in the MS spectra of blood, could be efficiently removed by Fe₃O₄@mTiO₂ based on the Lewis acid-Lewis base interactions. Following the optimization of the quantities of Fe₃O₄@mTiO₂, the method was applied to the determination of eight drugs in spiked blood. The analytical ranges typically extended from 2 to 500 ng mL^-1, and the recoveries ranged from 79.5-99.9% at different concentrations of blood. The limits of quantitation for drug poisons were 0.14-1.03 ng mL^-1, which makes the method a viable tool for drug poison monitoring in blood.

SUBMITTER: Yang F 

PROVIDER: S-EPMC9033689 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature