Enzyme cascade-amplified immunoassay based on the nanobody-alkaline phosphatase fusion and MnO₂ nanosheets for the detection of ochratoxin A in coffee.

ABSTRACT: Ochratoxin A (OTA) is a common food contaminant with multiple toxicities and thus rapid and accurate detection of OTA is indispensable to minimize the threat of OTA to public health. Herein a novel enzyme cascade-amplified immunoassay (ECAIA) based on the mutated nanobody-alkaline phosphatase fusion (mNb-AP) and MnO₂ nanosheets was established for detecting OTA in coffee. The detection principle is that the dual functional mNb-AP could specifically recognize OTA and dephosphorylate the ascorbic acid-2-phosphate (AAP) into ascorbic acid (AA), and the MnO₂ nanosheets mimicking the oxidase could be reduced by AA into Mn²⁺ and catalyze the 3,3',5,5'-tetramethyl benzidine into blue oxidized product for quantification. Using the optimal conditions, the ECAIA coul

SUBMITTER: Zhang Z 

PROVIDER: S-EPMC9034093 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature