Dataset Information

Multi-modal imaging for uptake of peptide ligand specific for CD44 by hepatocellular carcinoma.

ABSTRACT:

Background

Hepatocellular carcinoma (HCC) is rising steadily in incidence, and more effective methods are needed for early cancer detection and image-guided surgery.

Methods

We used a structural model to optimize the peptide sequence. Specific binding was validated in vitro with knockdown, competition, and co-localization assays. Multi-modal imaging was performed to validate specific binding in vivo in orthotopically-implanted human xenograft tumors.

Results

Binding properties of WKGWSYLWTQQA were characterized by an apparent dissociation constant of k_d = 43 nM, and an apparent association time constant of k = 0.26 min^-1. The target-to-background ratio was significantly higher for the target versus control for both modalities. Ex-vivo evaluation using human HCC specimens supported the ability of the peptide to distinguish HCC from other liver pathologies.

Conclusions

We have identified a peptide specific for CD44 with properties that are promising for clinical translation to image HCC in vivo.

SUBMITTER: Wu X

PROVIDER: S-EPMC9035732 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature

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Publications

Multi-modal imaging for uptake of peptide ligand specific for CD44 by hepatocellular carcinoma.

Wu Xiaoli X Meng Xiaoqing X Chang Tse-Shao TS Feng Shuo S Lee Miki M Jaiswal Sangeeta S Choi Eun-Young K EK Tran Lam L Jiang Hui H Wang Thomas D TD

Photoacoustics 20220411

<h4>Background</h4>Hepatocellular carcinoma (HCC) is rising steadily in incidence, and more effective methods are needed for early cancer detection and image-guided surgery.<h4>Methods</h4>We used a structural model to optimize the peptide sequence. Specific binding was validated in vitro with knockdown, competition, and co-localization assays. Multi-modal imaging was performed to validate specific binding in vivo in orthotopically-implanted human xenograft tumors.<h4>Results</h4>Binding propert ...[more]

PMID: 35479192

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Dataset Information

Multi-modal imaging for uptake of peptide ligand specific for CD44 by hepatocellular carcinoma.

Background

Methods

Results

Conclusions

Publications

Multi-modal imaging for uptake of peptide ligand specific for CD44 by hepatocellular carcinoma.

Similar Datasets

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