Multi-modal imaging for uptake of peptide ligand specific for CD44 by hepatocellular carcinoma.

ABSTRACT:

Background

Hepatocellular carcinoma (HCC) is rising steadily in incidence, and more effective methods are needed for early cancer detection and image-guided surgery.

Methods

We used a structural model to optimize the peptide sequence. Specific binding was validated in vitro with knockdown, competition, and co-localization assays. Multi-modal imaging was performed to validate specific binding in vivo in orthotopically-implanted human xenograft tumors.

Results

Binding properties of WKGWSYLWTQQA were characterized by an apparent dissociation constant of k_d = 43 nM, and an apparent association time constant of k = 0.26 min^-1. The target-to-background ratio was significantly higher for the target versus control for both modalities. Ex-vivo evaluation

SUBMITTER: Wu X 

PROVIDER: S-EPMC9035732 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature