Multiparameter immunohistochemistry analysis of HIV DNA, RNA and immune checkpoints in lymph node tissue.

ABSTRACT: The main barrier to a cure for HIV is the persistence of long-lived and proliferating latently infected CD4⁺ T-cells despite antiretroviral therapy (ART). Latency is well characterized in multiple CD4⁺ T-cell subsets, however, the contribution of regulatory T-cells (Tregs) expressing FoxP3 as well as immune checkpoints (ICs) PD-1 and CTLA-4 as targets for productive and latent HIV infection in people living with HIV on suppressive ART is less well defined. We used multiplex detection of HIV DNA and RNA with immunohistochemistry (mIHC) on formalin-fixed paraffin embedded (FFPE) cells to simultaneously detect HIV RNA and DNA and cellular markers. HIV DNA and RNA were detected by in situ hybridization (ISH) (RNA/DNAscope) and IHC was used to detect cellular markers (CD4,

SUBMITTER: Richardson ZA 

PROVIDER: S-EPMC9036546 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature