Usefulness of real-time RT-PCR to understand the kinetics of SARS-CoV-2 in blood: A prospective study.

ABSTRACT:

Background

SARS-CoV-2 viral load and kinetics assessed in serial blood samples from hospitalised COVID-19 patients by RT-PCR are poorly understood.

Methods

We conducted an observational, prospective case series study in hospitalised COVID-19 patients. Clinical outcome data (Intensive Care Unit admission and mortality) were collected from all patients until discharge. Viremia was determined longitudinally during hospitalisation, in plasma and serum samples collected sequentially, using two commercial and standardised RT-PCR techniques approved for use in diagnosis of SARS-CoV-2. Viral load (copies/mL and log10) was determined with quantitative TaqPath™COVID-19 test. Persistent viremia (PV) was defined as two or more consecutive quantifiable viral loads detected in blood sampl

SUBMITTER: Zurita-Cruz ND 

PROVIDER: S-EPMC9040491 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature