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A Dynamic Method for Broad-Spectrum Bacteriophage Cocktail Formulation Against Poultry-Associated Salmonella enterica.

ABSTRACT: Background: Poultry products are the largest food category linked to salmonellosis in Canada. Bacteriophages (phages) have been proposed as a novel antimicrobial in the poultry industry due to their documented ubiquity, efficacy, and safety benefits. Materials and Methods: A library of 78 lytic phages was rapidly screened against 50 prominent poultry-associated Salmonella enterica isolates procured from British Columbia, Canada. Results: A phage cocktail was successfully formulated using only three sewage-isolated phages (SE4, SE13, and SE20) to achieve broad-spectrum antimicrobial efficacy across all S. enterica serovars. Highly promising phages were also characterized using one-step growth curves and transmission electron microscopy. Conclusion: Relative host efficiency is a new agar-based semiquantitative metric developed here for the rapid comparison of different phages against a panel of known bacterial targets.

SUBMITTER: Brenner T 

PROVIDER: S-EPMC9041457 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature

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A Dynamic Method for Broad-Spectrum Bacteriophage Cocktail Formulation Against Poultry-Associated <i>Salmonella enterica</i>.

Brenner Thomas T   Fong Karen K   Lee Spencer S   Wang Siyun S  

PHAGE (New Rochelle, N.Y.) 20200616 2

<b><i>Background:</i></b> Poultry products are the largest food category linked to salmonellosis in Canada. Bacteriophages (phages) have been proposed as a novel antimicrobial in the poultry industry due to their documented ubiquity, efficacy, and safety benefits. <b><i>Materials and Methods:</i></b> A library of 78 lytic phages was rapidly screened against 50 prominent poultry-associated <i>Salmonella enterica</i> isolates procured from British Columbia, Canada. <b><i>Results:</i></b> A phage c  ...[more]

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