Galanin mediates tumor-induced immunosuppression in head and neck squamous cell carcinoma.

ABSTRACT:

Purpose

Galanin receptor 2 (GALR2) plays a significant role in the progression of head and neck squamous cell carcinomas (HNSCC). Since there is virtually no information on immunomodulation mediated by its ligand in the tumor microenvironment, we assessed the effects of galanin on peripheral blood mononuclear cells (PBMCs).

Methods

After verification of GALR2 expression and it activity in PBMCs we evaluated the effect of galanin and conditioned media from HNSCC cell lines silenced for galanin or antibody-depleted, on proliferation, apoptosis, cytokine expression and activation/differentiation of immune cells.

Results

We found that galanin alone and as a component of the HNSCC secretome decreased HNSCC cell proliferation and expression of pro-inflammatory cytokines (IF

SUBMITTER: de Medeiros MC 

PROVIDER: S-EPMC9050779 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature