Synthesis of 2,5-furandicarboxylic acid by a TEMPO/laccase system coupled with Pseudomonas putida KT2440.

ABSTRACT: As a useful and renewable chemical building block from biomass, 2,5-furandicarboxylic acid (FDCA) has become an increasingly desirable platform chemical as a terephthalic acid replacement for polymerization. In this work, an efficient and highly selective biocatalytic approach for the synthesis of FDCA from 5-hydroxymethylfurfural (HMF) was successfully developed using a TEMPO/laccase system coupled with Pseudomonas putida KT2440. TEMPO/laccase afforded the selective oxidation of the hydroxymethyl group of HMF to form 5-formyl-2-furancarboxylic acid as a major product, which was subsequently oxidized to FDCA by P. putida KT2440. Manipulating the reaction conditions resulted in a good conversion of HMF (100%) and an excellent selectivity of FDCA (100%) at substrate concentrati

SUBMITTER: Zou L 

PROVIDER: S-EPMC9054545 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature