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Lipoic acid capped silver nanoparticles: a facile route to covalent protein capping and oxidative stability within biological systems.

ABSTRACT: Covalent attachment of human serum albumin protein to the surface of spherical lipoic acid capped silver nanoparticles results in the generation of stable nanoparticle-protein hybrids with well defined surface composition. Enhanced stability towards oxidation and in the presence of complex media with high ionic strength, holds promise towards the use of these conjugates as therapeutics in biomedical applications and sensing.

SUBMITTER: Guzman-Soto I

PROVIDER: S-EPMC9056624 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature

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Lipoic acid capped silver nanoparticles: a facile route to covalent protein capping and oxidative stability within biological systems.

Guzmán-Soto Irene I Omole Mary M Alarcon Emilio I EI McTiernan Christopher D CD

RSC advances 20200904 54

Covalent attachment of human serum albumin protein to the surface of spherical lipoic acid capped silver nanoparticles results in the generation of stable nanoparticle-protein hybrids with well defined surface composition. Enhanced stability towards oxidation and in the presence of complex media with high ionic strength, holds promise towards the use of these conjugates as therapeutics in biomedical applications and sensing. ...[more]

PMID: 35516471

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Project description:Silver nanoparticles (AgNPs) are widely studied for their broad-spectrum antimicrobial effects, and can be utilised readily in biomaterials, however the cellular safety of specific AgNP formulations should be profiled prior to clinical usage. This study determined the cytotoxic effect of small sized (6 nm) alpha lipoic acid capped-AgNPs on human gingival fibroblasts (HGF), as compared to ionic silver and clinical antiseptics. The metabolic pathway was investigated to determine the cellular effects on HGF cells. The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) was established for a range of oral related bacteria. Results showed that cell viability decreased with increasing AgNP concentration, whereas lower concentrations of AgNPs, (≤5 μg ml-1) caused a significant increase in cell proliferation at 24 and 72 hour time points. The cytotoxicity profile of AgNPs exhibited significantly lower concentrations, relative to the dose of clinical efficacy, when compared to clinical antiseptics. Caspase 3/7 was not significantly altered when HGF cells were treated with 0.225 μg ml-1 AgNPs, indicating cell necrosis rather than apoptosis. Quantitative RT2-PCR detected an upregulation of genes associated with oxidative stress and the G2M cell cycle checkpoint at ≤4 hours, but expression levels returned to levels consistent with control cells at 24-96 hours. An MIC range of 2.5-12.5 μg ml-1 (min. Escherichia coli, Streptococcus mutans, S. mitis; max. Staphylococcus aureus) was determined across the bacterial species tested and an MBC range of 5-100 μg ml-1 (min. E. coli, max. S. mutans). The antimicrobial profile was similar to that of AgNO3 which suggested that the antimicrobial effect may be influenced by free Ag+ release. It was concluded that alpha lipoic acid capped AgNPs possess limited cytotoxic activity to HGF cells when compared to clinically utilised oral antiseptics, observed via the cellular recovery after initial AgNP treatment and a lack of cumulative cytotoxic effect, whilst maintaining a broad range antimicrobial effect of the AgNPs.

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Lipoic acid capped silver nanoparticles: a facile route to covalent protein capping and oxidative stability within biological systems.

Publications

Lipoic acid capped silver nanoparticles: a facile route to covalent protein capping and oxidative stability within biological systems.

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