Monitoring intracellular metal ion complexation with an acetylene-tagged ligand by Raman spectroscopy.

ABSTRACT: We propose to monitor molecular vibrations to identify metal ion-ligand complexation by means of Raman spectroscopy, which has been applied to track vibrational modes of molecules and to obtain a structural fingerprint. We prepared ligand molecules for Zn²⁺ ion complexation with a dipycolylaminoethyl aniline (DPEA) skeleton and phenylacetylene unit as the Raman tag which showed a typical band around 2200 cm^-1. Among the labeled ligands synthesized in this study, A-DPEA showed a strong band attributed to the acetylene unit at 2212 cm^-1, while the addition of Zn²⁺ ion resulted in a band shift to 2220 cm^-1 due to complex formation. The addition of other metal ions and titration experiments showed that A-DPEA bound with Zn²⁺ sel

SUBMITTER: Takemura S 

PROVIDER: S-EPMC9056985 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature