Isolation, expression, and biochemical characterization: nitrite reductase from Bacillus cereus LJ01.

ABSTRACT: Biological remediation of toxic oxygen-containing anions such as nitrate that are common in the environment is of great significance. Therefore, it is necessary to understand the specific role of nitrate and nitrite reductase in the bioremediation process. Bacillus cereus LJ01, which was isolated from traditional Chinese soybean paste, effectively degraded nitrite (such as NaNO₂) at 0-15 mmol L^-1 in LB medium. Moreover, the nitrite-degrading active substance (ASDN) was isolated and purified from B. cereus LJ01. The nitrite-degrading activity of nitrite reductase (named LJ01-NiR) was 4004.89 U mg^-1. The gene encoding the assimilation of nitrite reductase in B. cereus LJ01 was cloned and overexpressed in E. coli. The purified recom

SUBMITTER: Huang YY 

PROVIDER: S-EPMC9057199 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature