Single-sister labeling in the C. elegans germline using the nucleotide analog EdU.
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ABSTRACT:
SUBMITTER: Almanzar DE
PROVIDER: S-EPMC9059099 | biostudies-literature | 2022 Jun
REPOSITORIES: biostudies-literature
Publications
Single-sister labeling in the <i>C. elegans</i> germline using the nucleotide analog EdU.
Almanzar David E DE Hamrick Antonia A Rog Ofer O
STAR protocols 20220423 2
Reciprocal exchanges between genetically identical sister chromatids (sister chromatid exchanges or SCEs) have been challenging to study. Here, we describe a protocol that utilizes a pulse/chase of the thymidine analog 5-ethyl-3'-deoxyuridine (EdU) in combination with click chemistry and antibody labeling to selectively label sister chromatids in the <i>C. elegans</i> germline. Labeling has no discernable effects on meiosis, allowing for cytological quantification of SCEs. This protocol can be c ...[more]
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