FoxP3 associates with enhancer-promoter loops to regulate T_reg-specific gene expression.

ABSTRACT: Gene expression programs are specified by higher-order chromatin structure and enhancer-promoter loops (EPLs). T regulatory cell (T_reg) identity is dominantly specified by the transcription factor (TF) FoxP3, whose mechanism of action is unclear. We applied chromatin conformation capture with immunoprecipitation (HiChIP) in T_reg and closely related conventional CD4⁺ T cells (T_conv). EPLs identified by H3K27Ac HiChIP showed a range of connection intensity, with some superconnected genes. TF-specific HiChIP showed that FoxP3 interacts with EPLs at a large number of genes, including some not differentially expressed in T_reg versus T_conv, but enriched at the core T_reg signature loci that it up-regulates. FoxP3 associa

SUBMITTER: Ramirez RN 

PROVIDER: S-EPMC9059705 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature