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Functional enzyme-polymer complexes.

ABSTRACT: SignificanceThe use of biological enzyme catalysts could have huge ramifications for chemical industries. However, these enzymes are often inactive in nonbiological conditions, such as high temperatures, present in industrial settings. Here, we show that the enzyme PETase (polyethylene terephthalate [PET]), with potential application in plastic recycling, is stabilized at elevated temperature through complexation with random copolymers. We demonstrate this through simulations and experiments on different types of substrates. Our simulations also provide strategies for designing more enzymatically active complexes by altering polymer composition and enzyme charge distribution.

SUBMITTER: Waltmann C

PROVIDER: S-EPMC9060439 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature

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Functional enzyme-polymer complexes.

Waltmann Curt C Mills Carolyn E CE Wang Jeremy J Qiao Baofu B Torkelson John M JM Tullman-Ercek Danielle D Olvera de la Cruz Monica M

Proceedings of the National Academy of Sciences of the United States of America 20220321 13

SignificanceThe use of biological enzyme catalysts could have huge ramifications for chemical industries. However, these enzymes are often inactive in nonbiological conditions, such as high temperatures, present in industrial settings. Here, we show that the enzyme PETase (polyethylene terephthalate [PET]), with potential application in plastic recycling, is stabilized at elevated temperature through complexation with random copolymers. We demonstrate this through simulations and experiments on ...[more]

PMID: 35312375

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