Alterations in 3D chromatin organization contribute to tumorigenesis of EGFR-amplified glioblastoma.

ABSTRACT:

Background

EGFR amplification and/or mutation are found in more than half of the cases with glioblastoma. Yet, the role of chromatin interactions and its regulation of gene expression in EGFR-amplified glioblastoma remains unclear.

Methods

In this study, we explored alterations in 3D chromatin organization of EGFR-amplified glioblastoma and its subsequent impact by performing a comparative analysis of Hi-C, RNA-seq, and whole-genome sequencing (WGS) on EGFR-amplified glioblastoma-derived A172 and normal astrocytes (HA1800 cell line).

Results

A172 cells showed an elevated chromatin relaxation, and unexpected entanglement of chromosome regions. A genome-wide landscape of switched compartments and differentially expressed genes between HA1800

SUBMITTER: Yang Q 

PROVIDER: S-EPMC9062087 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature