The CLIP-170 N-terminal domain binds directly to both F-actin and microtubules in a mutually exclusive manner.

ABSTRACT: The cooperation between the actin and microtubule (MT) cytoskeletons is important for cellular processes such as cell migration and muscle cell development. However, a full understanding of how this cooperation occurs has yet to be sufficiently developed. The MT plus-end tracking protein CLIP-170 has been implicated in this actin-MT coordination by associating with the actin-binding signaling protein IQGAP1 and by promoting actin polymerization through binding with formins. Thus far, the interactions of CLIP-170 with actin were assumed to be indirect. Here, we demonstrate using high-speed cosedimentation assays that CLIP-170 can bind to filamentous actin (F-actin) directly. We found that the affinity of this binding is relatively weak but strong enough to be significant in the actin-rich c

SUBMITTER: Wu YO 

PROVIDER: S-EPMC9062740 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature