NAD⁺-boosting molecules suppress mast cell degranulation and anaphylactic responses in mice.

ABSTRACT: Nicotinamide adenine dinucleotide (NAD⁺) acts as a cofactor for multiple biological processes. While previous research has revealed that the NAD⁺ declines associated with aging contributes to an impairment of immune cells, its role in mast cell function, especially in response to an anaphylactic condition, has remained unexplored. We tested whether the restoration of cellular NAD⁺ concentration by the supplementation of NAD⁺ boosting molecules prevented mast cell degranulation and anaphylactic responses. Methods: Bone marrow derived mast cells (BMMCs) and human cord blood derived mast cells were treated with NAD⁺ precursors nicotinamide mononucleotide (NMN) and nicotinamide riboside (NR), and FcεRI downstream signaling was asses

SUBMITTER: Kim HW 

PROVIDER: S-EPMC9065190 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature