Combining rapid diagnostic tests to estimate primary and post-primary dengue immune status at the point of care.

ABSTRACT:

Background

Characterising dengue virus (DENV) infection history at the point of care is challenging as it relies on intensive laboratory techniques. We investigated how combining different rapid diagnostic tests (RDTs) can be used to accurately determine the primary and post-primary DENV immune status of reporting patients during diagnosis.

Methods and findings

Serum from cross-sectional surveys of acute suspected dengue patients in Indonesia (N:200) and Vietnam (N: 1,217) were assayed using dengue laboratory assays and RDTs. Using logistic regression modelling, we determined the probability of being DENV NS1, IgM and IgG RDT positive according to corresponding laboratory viremia, IgM and IgG ELISA metrics. Laboratory test thresholds for RDT positivity/negativity were calcul

SUBMITTER: Biggs JR 

PROVIDER: S-EPMC9067681 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature