Early and Rapid Identification of COVID-19 Patients with Neutralizing Type I Interferon Auto-antibodies.

ABSTRACT:

Purpose

Six to 19% of critically ill COVID-19 patients display circulating auto-antibodies against type I interferons (IFN-AABs). Here, we establish a clinically applicable strategy for early identification of IFN-AAB-positive patients for potential subsequent clinical interventions.

Methods

We analyzed sera of 430 COVID-19 patients from four hospitals for presence of IFN-AABs by ELISA. Binding specificity and neutralizing activity were evaluated via competition assay and virus-infection-based neutralization assay. We defined clinical parameters associated with IFN-AAB positivity. In a subgroup of critically ill patients, we analyzed effects of therapeutic plasma exchange (TPE) on the levels of IFN-AABs, SARS-CoV-2 antibodies and clinical outcome.

Results

The prevalen

SUBMITTER: Akbil B 

PROVIDER: S-EPMC9069123 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature