Symmetric exponential amplification reaction-based DNA nanomachine for the fluorescent detection of nucleic acids.

ABSTRACT: By introducing palindromic sequences into the classical exponential amplification reaction (EXPAR), we constructed a new palindromic fragment-incorporated multifunctional hairpin probe (P-HP)-mediated symmetric exponential amplification reaction (S-EXPAR), to significantly reduce the background signal caused by inherent nonspecific amplification. A G-triplex/ThT complex was used as the signal reporter for the proposed label-free DNA nanomachine. The P-HP consists of five functional regions: a C-rich region (C), a target DNA recognition region (T'), two nicking sites (X') and a palindromic fragment (P). When target DNA (T) hybridizes with P-HP, the palindromic fragment at the 3' end of P-HP is fully exposed. Then, the P-HP/T duplexes hybridize with each other through the exposed P, and EXPA

SUBMITTER: Yan Q 

PROVIDER: S-EPMC9076420 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature