Engineering stem cell-derived 3D brain organoids in a perfusable organ-on-a-chip system.

ABSTRACT: Brain organoids derived from the self-organization of human induced pluripotent stem cells (hiPSCs) represent a new class of in vitro organ system for modeling brain development and diseases. However, engineering brain organoids in a biomimetic environment that is favorable for brain development remains challenging. In this work, we present a new strategy to generate hiPSCs-derived 3D brain organoids using an organ-on-a-chip system in a controlled manner. This system provides a biomimetic brain microenvironment by incorporating three-dimensional (3D) Matrigel, fluid flow and multicellular architectures of tissues that allows for extended 3D culture, in situ neural differentiation, and organization of brain organoids on a single device. The generated brain organoids display we

SUBMITTER: Wang Y 

PROVIDER: S-EPMC9077091 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature