Efficient promiscuous Knoevenagel condensation catalyzed by papain confined in Cu₃(PO₄)₂ nanoflowers.

ABSTRACT: To develop an efficient and green immobilized biocatalyst for promiscuous catalysis which has a broad scope of applications, hybrid nanoflower (hNF) confined papain as a biocatalyst has been proposed and characterized in this study. hNFs were firstly prepared through mixing CuSO₄ aqueous solution with papain in phosphate saline (PBS) at room temperature. The resulting hNFs were characterized by SEM and verified through a hydrolysis reaction with N-benzoyl-dl-arginine amide as substrate. Under optimal conditions, this nano-biocatalyst demonstrated a 15-fold hydrolytic activity compared with papain of free form, along with better thermal stability. A series of reaction factors (reaction temperature, time, and solvent) have been investigated for Knoevenagel condensation reac

SUBMITTER: Yu J 

PROVIDER: S-EPMC9077389 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature