Thiol-ene photoimmobilization of chymotrypsin on polysiloxane gels for enzymatic peptide synthesis.

ABSTRACT: Chemical incorporation of enzymes onto polymeric materials has recently attracted intense scientific attention. Cross-linked polysiloxane gels as a typical super-hydrophobic support, are a good candidate for supporting enzymes in low-water organic medium to efficiently catalyze peptide synthesis because the hydrophobic polysiloxane matrix can prevent water from attacking the acyl-enzyme intermediate, which is beneficial for the shift in equilibrium to peptide formation. In this work, we develop a facile strategy to photoimmobilize olefin-functionalized chymotrypsin onto cross-linked polysiloxane gels via UV-initiated thiol-ene click chemistry. The impacts of water addition amount, heat-treatment and recyclability of the immobilized chymotrypsin influencing the peptide synthesis effi

SUBMITTER: Wang M 

PROVIDER: S-EPMC9079220 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature