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Novel caged luciferin derivatives can prolong bioluminescence imaging in vitro and in vivo.

ABSTRACT: Based on N-cyclobutylaminoluciferin (cybLuc), a set of high and efficient caged bioluminescent derivatives (Clucs) as firefly luciferase pro-substrates has been developed herein. After careful examination, these molecules exhibited low cytotoxicity and prolonged bioluminescence imaging up to 6 h in vitro and in vivo. Importantly, these caged luciferin derivatives have the potential to serve as long-term tracking tools to explore some biological process by using bioluminescent imaging.

SUBMITTER: Zhang C

PROVIDER: S-EPMC9080648 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature

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Novel caged luciferin derivatives can prolong bioluminescence imaging in vitro and in vivo.

Zhang Chaochao C Yuan Mingliang M Han Guangxi G Gao Yuqi Y Tang Chunchao C Li Xiang X Du Lupei L Li Minyong M

RSC advances 20180530 35

Based on N-cyclobutylaminoluciferin (cybLuc), a set of high and efficient caged bioluminescent derivatives (Clucs) as firefly luciferase pro-substrates has been developed herein. After careful examination, these molecules exhibited low cytotoxicity and prolonged bioluminescence imaging up to 6 h in vitro and in vivo. Importantly, these caged luciferin derivatives have the potential to serve as long-term tracking tools to explore some biological process by using bioluminescen ...[more]

PMID: 35540985

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