Comparative metabolomics analysis of bronchial epithelium during barrier establishment after allergen exposure.

ABSTRACT:

Background

Several studies have shown a correlation between an altered metabolome and respiratory allergies. The epithelial barrier hypothesis proposes that an epithelial barrier dysfunction can result in allergic diseases development. Der p 1 allergen from house dust mite is a renowned epithelial barrier disruptor and allergy initiator due to its cysteine-protease activity. Here, we compared the metabolic profile of the bronchial epithelium exposed or not to Der p 1 during barrier establishment to understand its active role in allergy development.

Methods

Calu-3 cells were cultivated in air-liquid interface cultures and exposed to either Der p 1 or Ole e 1 allergens during barrier establishment. The comparative metabolomics analysis of apical and basolateral media were perf

SUBMITTER: Lopez-Rodriguez JC 

PROVIDER: S-EPMC9082991 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature