A negative-solvatochromic fluorescent probe for visualizing intracellular distributions of fatty acid metabolites.

ABSTRACT: Metabolic distribution of fatty acid to organelles is an essential biological process for energy homeostasis as well as for the maintenance of membrane integrity, and the metabolic pathways are strictly regulated in response to environmental stimuli. Herein, we report a fluorescent fatty acid probe, which bears an azapyrene dye that changes its absorption and emission features depending on the microenvironment polarity of the organelle into which it is transported. Owing to the environmental sensitivity of this dye, the distribution of the metabolically incorporated probe in non-polar lipid droplets, medium-polarity membranes, and the polar aqueous regions, can be visualized in different colors. Based on density scatter plots of the fluorophore, we demonstrate that the degradation of triac

SUBMITTER: Kajiwara K 

PROVIDER: S-EPMC9085894 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature